Journal: bioRxiv

Article Title: Uncovering a new player in ischemic stroke: a study of intra-arterial interferon-gamma-producing monocytes in hyperacute stroke

doi: 10.1101/2025.08.19.671149

Figure Lengend Snippet: A ) Representative flow cytometry plots (left) and quantification (center and right) of IFN-γ + cells within CD4 + and CD8 + T-cell populations from pre- and post-occlusion arterial blood samples. No significant differences were observed between conditions, and paired analysis revealed no consistent relationship, suggesting CD4 + and CD8 + T-cells are not the primary contributors to increased IFN-γ levels during the hyperacute phase. B ) IFN-γ expression in non-classical IFN-γ producing cell subsets, including CD11c + dendritic cells and CD19 + B-cells. Quantification revealed no significant changes in the percentage of IFN-γ + cells in either population. C ) In contrast, IFN-γ + cells within the CD14 + population were significantly increased in post-occlusion relative to pre-occlusion samples. Paired animal analysis confirmed this consistent increase across samples, identifying CD14 + monocytes as a major source of IFN-γ in the immediate post-ictus period. The data are presented as the means ± SD. Each dot represents an individual animal (n=19/group). Significance was determined by paired or unpaired Student’s t test as appropriate. *p< 0.05, ****p< 0.0001.

Article Snippet: The following fluorochrome-conjugated monoclonal antibodies were added to the samples: anti-mouse IFN-γ violetFluor450 (Cat. 75-7311-U100, Tonbo Biosciences, CA), anti-mouse CD3ε BV711 (Cat. 100348, BD Biosciences, NJ), anti-mouse CD11c FITC (Cat. 35-0114-U025, Tonbo Biosciences, CA), anti-mouse CD19 PE (Cat. 50-0193-U100, Tonbo Biosciences, CA), anti-mouse CD8a PE-Cy5 (Cat. 55-0081-U25, Tonbo Biosciences, CA), anti-mouse CD25 PE-Cy7 (Cat. 60-025-U100, Tonbo Biosciences, CA), anti-mouse CD14 APC (Cat. 123312, BioLegend, CA), anti-mouse CD4 redFluor 710 (Cat. 80-0042-U100, Tonbo Biosciences, CA), anti-mouse CD69 APC (Cat. 20-0691-U025, Tonbo Biosciences, CA), anti-mouse IL-4 PE (Cat. 50- 7041-U025, Tonbo Biosciences, CA), and anti-mouse IL-17A FITC (Cat. 506907, BioLegend, CA).

Techniques: Flow Cytometry, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Characterization and angiogenic potential of CD146 + endometrial stem cells

doi: 10.1186/s13287-024-03918-7

Figure Lengend Snippet: A variety of cell surface markers are employed for the marker study of eSCs. Conjugated antibodies were used to conduct the experiment. The antibody concentrations were adjusted according to the specific recommendation for each antibody

Article Snippet: 17. , CD19 PE conjugate , IgG1 , Mouse , 10 μL/10^6 cells , R&D Systems, FAB4867P.

Techniques: Marker

Journal: Stem Cell Research & Therapy

Article Title: Characterization and angiogenic potential of CD146 + endometrial stem cells

doi: 10.1186/s13287-024-03918-7

Figure Lengend Snippet: Isolation and culturing eSCs, surface marker analysis and growth factor analysis Figure 1 A ; The sample was collected from the hospital (i), trypsin was used to digest it (ii), centrifugation was used to obtain the cell pellet (iii), and the isolated cells were cultured in full DMEM media (iv) image is taken at 10X. Figure 1 B ; The endometrial cells showed remarkable expression of mesenchymal (CD90, CD105, CD73, CD140b, CD146), cell adhesion molecules (CD29, CD44, CD166). However, the expression of HLA-DR, CD34, CD45, CD14 and CD19 were negative. Figure 1 C ; In growth factor secretion profile GM-CSF, G-CSF secretion concentration was higher than others. Followed by EGF, FGF basic, PDGF-AA and VEGF ( p value: <0.0001)

Article Snippet: 17. , CD19 PE conjugate , IgG1 , Mouse , 10 μL/10^6 cells , R&D Systems, FAB4867P.

Techniques: Isolation, Marker, Centrifugation, Cell Culture, Expressing, Concentration Assay

Journal: Molecular therapy. Methods & clinical development

Article Title: Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus.

doi: 10.1016/j.omtm.2022.05.006

Figure Lengend Snippet: Figure 3. Immunophenotyping of 1D3-CD19CAR- GFP (blue, circle), FMC63-CD19CAR-GFP (red, square), or control (GFP only, green, triangle) trans- duced T cells in peripheral blood of treated mice Values shown are the percentage of total CD3+, CD90.2+

Article Snippet: In order to verify the ability for both the 1D3-and FMC63-CD19CAR to target the mouse CD19 antigen, the A20 cells were incubated with 10, 1, 0.1, 0.001, or 0 mg/mL of either the 1D3CD19-blocking antibody (152402, BioLegend) or the FMC63-CD19- Molecular Th blocking antibody (NBP2-52716, Novus Biologicals).

Techniques: Control

Journal: bioRxiv

Article Title: Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to vaccination

doi: 10.1101/2023.05.23.541927

Figure Lengend Snippet: Transcriptional features of ST3-reactive and non-ST3-reactive B cells elicited by vaccination. A) Representative staining of ST3-reactive B cells from unvaccinated and vaccinated samples, gated on IgG+ B cells (Live CD3 - CD19 + CD20 + IgG + ). B) Frequency of ST3-reactive IgG+ B cells in vaccinated and unvaccinated monkeys. C, D) Correlation between ST3-reactive IgG+ B cells and ST3-specific IgG (C) and OPA (D) titers. E, F) UMAP of single-cell transcriptomes colored by sort fraction (E) and cell phenotype (F). Dot plot showing scaled expression and percent of cells expressing B cell marker genes in each transcriptional phenotype. H) Volcano plot of differentially expressed transcripts between B2M hi and MBC-like cells. P-values are calculated using a two-sided Wilcoxon rank-sum test and are adjusted using Bonferroni correction.

Article Snippet: Cells were then washed three times in FBS staining buffer (BD) and were then stained with BV605-conjugated anti-IgM (MHM-88), BV786-conjugated anti-CD20 (both from Biolegend), DyLight 405-congjuatated anti-CD19 (CB19) (R&D systems), V500-conjugated CD3 (SP34-2), PE-Cy5-conjugated anti-IgG (G18-145) (both from BD), and Total-seq A anti-human hashtag antibody (Biolegend) on ice for 30 minutes.

Techniques: Staining, Expressing, Marker