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Image Search Results
Journal: Molecular therapy. Methods & clinical development
Article Title: Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus.
doi: 10.1016/j.omtm.2022.05.006
Figure Lengend Snippet: Figure 3. Immunophenotyping of 1D3-CD19CAR- GFP (blue, circle), FMC63-CD19CAR-GFP (red, square), or control (GFP only, green, triangle) trans- duced T cells in peripheral blood of treated mice Values shown are the percentage of total CD3+, CD90.2+
Article Snippet: In order to verify the ability for both the 1D3-and FMC63-CD19CAR to target the mouse CD19 antigen, the A20 cells were incubated with 10, 1, 0.1, 0.001, or 0 mg/mL of either the 1D3CD19-blocking antibody (152402, BioLegend) or the
Techniques: Control
Journal: Experimental & molecular medicine
Article Title: Distribution and impact of p16 INK4A+ senescent cells in elderly tissues: a focus on senescent immune cell and epithelial dysfunction.
doi: 10.1038/s12276-024-01354-4
Figure Lengend Snippet: Fig. 4 The distribution of CDKN2A+ cells in normal lung and liver tissues from young and aged individuals in scRNA-seq. a Feature plots display the expression of CDKN2A in the public dataset of lung, SYN1041850. b Bar graphs depict the proportion of CDKN2A+ cells in each cell type of the lung tissue. c The multiplex IHC analysis shows each subtype of CD19+ B cells according to the p16INK4A expression. d Feature plots display the expression of CDKN2A in the public dataset of liver, GSE149614. e The quantification data of CDKN2A+ cells in each cell type of liver are shown. f The multiplex IHC analysis shows each subtype of CD68+ Kupffer cells according to the p16INK4A expression.
Article Snippet: The primary antibodies used for human samples were as follows: p16INK4A, predilution (805–4713, Roche, Basal, Switzerland); human Ki67, 1:3,000 (M7240, Dako, Glostrup, Denmark); H3K9me3, 1:500 (ab176916, Abcam, Cambridge, MA); CD3, 1:150 (ab135372, Abcam);
Techniques: Expressing, Multiplex Assay